The effects of basal media and plant growth regulators on the microshoots proliferation and callus induction of cranberry (Vaccinum. arctostaphylos L.) explants

Document Type : Original Article

Authors

1 M.Sc. Student, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

3 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

4 M.Sc. Graduate, Imam Khomeini International University, Qazvin, Iran.

5 Instructor, Department of Agriculture, Faculty of Sciences, University of Gonabad, Gonabad, Iran.

10.30466/rip.2022.52577.1094

Abstract

Cranberry (Vaccinium arctostaphylos L.) is the only shrub species of the genus Vaccinium in Iran and grows in northern forests. This study was carried out to evaluate the effect of different basal culture media and growth regulators on the establishment, proliferation and callus induction of cranberry micro-shoots under in vitro conditions. In the proliferation of microshoots, three types of basal culture media, AN, QL and ½ MS and four concentrations of zeatin, 0 (control), 1, 2 and 3 mg/L, along with a fixed concentration of 0.2 mg/L of indole acetic acid (IAA) in each basal culture media were used in all replicates. The experiment was carried out as a factorial in a completely randomized basic design with 4 replications. For the callus formation of microshoots, the basal culture media ½ QL with two concentrations (10 and 20 μM) of zeatin, TDZ and BAP, along with three concentrations of NAA (1, 5 and 10 μM) were used in a completely randomized experimental design with five replications. In all experiments, some parameters such as shoot length, number of shoots, the number of nodes and leaves formed, the percentage and the number of days to callus formation were evaluated. The results showed that the number of shoots in explants grown in the basal medium did not differ significantly, while the shoot length of explants was higher in the AN basal medium compared to other basal media. However, the type of basal medium showed significant effects on the number of leaves, and the AN basal medium had the highest number of leaves. While the ½ MS and AN basal media, in terms of the number of nodes, performed better than QL. The concentration of 3 mg/L of zeatin in ½ MS basal medium had the best performance in shoot length, and 1 mg/L of zeatin in AN basal medium had the best performance in terms of shoot number, leaf number and node number. In the induction of callus formation, zeatin had the best performance, and BAP was the most efficient growth regulator in terms of callus formation time.

Keywords

References

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Research in Pomology
Volume 7, Issue 1 - Serial Number 1
September 2022
Pages 68-79

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