Effect of a combined edible coating of beeswax, chitosan, and cinnamon nanoemulsion with salicylic acid on the storage life and postharvest quality of strawberry (Fragaria × ananassa)

Document Type : Original Article

Authors

1 M.Sc. Student, Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Assistant Professor, Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Professor, Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

10.30466/rip.2025.56089.1347

Abstract

The use of edible coatings is an appropriate strategy for extending the marketability of strawberries. This study was conducted to investigate the effects of edible coatings on the quantitative and qualitative characteristics of strawberry cv. Albion during storage using a factorial experiment based on a completely randomized design. The edible coating factor consisted of nine levels, and the evaluation time factor consisted of four levels. The results showed that the use of edible coatings reduced fruit weight loss compared with the control, with weight loss reaching 29.18% in the control treatment and only 0.11% of the initial fruit weight in the treatment containing 1.5% chitosan + 10% beeswax + 2‰ cinnamon nanoemulsion. Fruits treated with cinnamon nanoemulsion at a concentration of 2‰ exhibited the longest storage life (mean of 8.12 days). Increasing the concentration of the essential oil and nanoemulsion improved fruit quality, resulting in increases in anthocyanin content (43.5%), titratable acidity (2.12-fold), total antioxidant capacity (60.81%), and acidity (2.83-fold) compared with the control. Storage time reduced fruit firmness and visual quality. The results showed that treating strawberry fruit with different edible coatings, particularly the pre-storage application of 1.5% chitosan + 10% beeswax + 2‰ cinnamon nanoemulsion to strawberry cv. Albion, had positive effects on maintaining the quantitative and qualitative characteristics of the fruit, increasing its storage life by 62.02% compared with the control. Therefore, the use of edible coatings is recommended for improving the storage characteristics of strawberry cv. Albion.

Keywords


جایمند، ک. و رضایی، م.ب. 1390. دستگاه‏های تقطیر، روش‏های آزمون و شاخص‏های بازداری در تجزیه اسانس. انجمن گیاهان دارویی، تهران. 350 ص.
جلیلی‏مرندی، ر. 1391. فیزیولوژی بعد از برداشت (جابجایی و نگهداری میوه، سبزی، گیاهان زینتی و گیاهان دارویی). انتشارات جهاد دانشگاهی، ارومیه. 624 ص.
جهان‏تیغی، ف.، قلی‏پورکنعانی، ح.، ابراهیمی، پ.، جعفریان، ح. و شاهسونی، د. 1395. استفاده از اسانس میخک و نانواسانس میخک (Eugenia caryophillata) در بیهوشی ماهی قزل‏آلای رنگین کمان (Oncorhynchus mykiss). همایش ملی گیاهان دارویی معطر و ادویه‏ای.
حسینی­فرهی، م.، رادی، م.، باقری، ف. و جمشیدی، ا. 1397. بررسی ویژگی‏های کیفی و ارگانولپتیکی پس از برداشت میوه توت فرنگی با کاربرد ژل آلوئه‏ورا، استیک اسید و پرتو فرابنفش بی. مجله علوم و فنون باغبانی ایران، 19(1): 114-99.
دهقانی، ب.، قنبری‏جهرمی، م. و سعیدی‌سار، س. 1400. تأثیر اسانس آویشن شیرازی (Zataria multiflora boiss.) بر کیفیت انبارمانی میوه گلابی (Pyrus communis) رقم ‘Green anjou’. پژوهش‏های میوه‏کاری، 6(1): 147-129.
سلیمی، ف. 1395. مروری بر خواص دارویی و ترکیبات شیمیایی گیاه توت­فرنگی. اولین سمپوزیوم ملی میوه‏های ریز، 471-475.
عشقی، س.، هاشمی، م.، محمدی، ع.، بدیعی، ف.، محمدحسینی، ز.، احمدی‏صومعه، ک. و قناتی، ک. 1392. تأثیر پوشش نانوامولسیون حاوی کیتوزان بر افزایش ماندگاری و ویژگی‏های کیفی میوه توت­فرنگی پس از برداشت. مجله علوم تغذیه و صنایع غذایی ایران، 2(8): 9-19.
قاسمی‏تولائی، م.، رامین، ع.ا. و امینی، ف. 1394. تأثیر پوشش خوراکی کیتوزان بر کیفیت و افزایش عمر پس از برداشت خیار رقم زمرد. نشریه تولید و فرآوری محصولات زراعی و باغی، ۵(۱۵): ۱98-۱89.
مستوفی، ی. و نجفی، ف. 1384. روش‏های آزمایشگاهی تجزیه‏ای در علوم باغبانی. انتشارات دانشگاه تهران (ترجمه). 136 ص.
میرجلیلی، س.ع. و پیرو، ح. 1400. بررسی تأثیر اسیدسالیسیلیک بر افزایش عمرانباری میوه انگور Vitis vinifera. اولین همایش ملی گیاهان دارویی، کارآفرینی و تجاری‏سازی، جیرفت.
نصرالله­زاده‏اصل، ن. 1392. تأثیر پوشش‏های خوراکی در حفظ کیفیت و افزایش ماندگاری میوه‏ها و سبزی‏ها. فصلنامه نظام مهندسی کشاورزی و منابع طبیعی، 11(42): 31.
Al-Rashdi, S., Al-Subhi, N., Al-Dairi, M. and Pathare, P.B. 2024. Effect of a Moringa oil–Beeswax edible coating on the shelf-life and quality of fresh cucumber. Processes, 12(6): 1148.
Baswal, A.K., Dhaliwal, H.S., Singh, Z., Mahajan, B.V.C., Kalia, A. and Gill, K.S. 2020. Influence of carboxy methylcellulose, chitosan and beeswax coatings on cold storage life and quality of Kinnow mandarin fruit. Scientia Horticulturae, 260: 108887.
Carole, D.A., Derricka, M.D.R.T., Dé, M.H., Souaibou, A., Cé, M.E., Joë, B.N., Dioril, A.D., Didier, M.B., Charlen, N.I.B., Emmanuel, K. and Xavier, K.F.A. 2024. Strawberry (Fragaria spp.): cultivation, production, consumption, and marketing in cameroon. Agricultural Sciences, 15(4): 449-471.
Carpena, M., Nuñez-Estevez, B., Soria-Lopez, A., Garcia-Oliveira, P. and Prieto, M.A. 2021. Essential oils and their application on active packaging systems: A review. Resources, 10(1): 7.
De Bruno, A., Gattuso, A., Ritorto, D., Piscopo, A. and Poiana, M. 2023. Effect of edible coating enriched with natural antioxidant extract and bergamot essential oil on the shelf life of strawberries. Foods, 12(3): 488.
Dong, F. and Wang, X. 2017. Effects of carboxymethyl cellulose incorporated with garlic essential oil composite coatings for improving quality of strawberries. International Journal of Biological Macromolecules, 104: 821-826.
Du, W.X., Olsen, C.W., Avena Bustillos, R.J., McHugh, T.H., Levin, C.E. and Friedman, M. 2009. Effects of allspice, cinnamon, and clove bud essential oils in edible apple films on physical properties and antimicrobial activities. Journal of Food Science, 74(7): M372-M378.
Efendi, D. and Hermawati, H. 2010. The use of bee wax, chitosan and BAP to prolong shelflife of Mangosteen (Garcinia mangostana L.) fruit. Jurnal Hortikultura Indonesia (JHI), 1(1): 32-39.
Eshetu, A., Ibrahim, A.M., Forsido, S.F. and Kuyu, C.G. 2019. Effect of beeswax and chitosan treatments on quality and shelf life of selected mango (Mangifera indica L.) cultivars. Heliyon, 5(1): e01116.
Gonzalez, E.M., de Ancos, B. and Cano, M.P. 1999. Partial characterization of polyphenol oxidase activity in raspberry fruits. Journal of Agricultural and Food Chemistry, 47(10): 4068-4072.
Han, Y., Yu, M. and Wang, L. 2018. Physical and antimicrobial properties of sodium alginate/carboxymethyl cellulose films incorporated with cinnamon essential oil. Food Packaging and Shelf Life, 15: 35-42.
Hesami, G., Darvishi, S., Zarei, M. and Hadidi, M. 2021. Fabrication of chitosan nanoparticles incorporated with Pistacia atlantica subsp. kurdica hulls’ essential oil as a potential antifungal preservative against strawberry grey mould. International Journal of Food Science and Technology, 56(9): 4215-4223.
Horison, R., Sulaiman, F.O., Alfredo, D. and Wardana, A.A. 2019. Physical characteristics of nanoemulsion from chitosan/nutmeg seed oil and evaluation of its coating against microbial growth on strawberry. Food Research, 3(6): 821-827.
Lee, J., Durst, R.W. and Wrolstad, R.E. 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC International, 88(5): 1269-1278.
Lin, M.G., Lasekan, O., Lasekan, O.O., Nazamid, S. and Bejo, S.K. 2018. Effect of chitosan and carrageenan-based edible coatings on post-harvested longan (Dimocarpus longan) fruits. CYTA - Journal of Food, 16(1): 490-497.
Liu, J., Liu, Y., Shao, S., Zheng, X. and Tang, K. 2022. Soluble soybean polysaccharide/carboxymethyl chitosan coatings incorporated with lavender essential oil: Structure, properties and application in fruit preservation. Progress in Organic Coatings, 173: 107178.
Márquez-Villacorta, L., Pretell-Vásquez, C. and Hayayumi-Valdivia, M. 2022. Optimization of edible coating with essential oils in blueberries. Ciencia e Agrotecnologia, 46: e006022.
Martínez, K., Ortiz, M., Albis, A., Gilma Gutiérrez Castañeda, C., Valencia, M.E. and Grande Tovar, C.D. 2018. The effect of edible chitosan coatings incorporated with Thymus capitatus essential oil on the shelf-life of strawberry (Fragaria x ananassa) during cold storage. Biomolecules, 8(4): 155.
McDonald, S., Prenzler, P.D., Antolovich, M. and Robards, K. 2001. Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73(1): 73-84.
Muddather, A.E. and Abu-Goukh, A.B.A. 2019. Effect of Maleic Hydrazide and Waxing on Quality and Shelf-life of Mango (Mangifera indica L.) Fruits. University of Khartoum Journal of Agricultural Sciences, 24(1): 59-72.
Mukdisari, Y., Suketi, K. and Widodo, W.D. 2016. Fruit coating with chitosan and beeswax to extend papaya shelf life. Journal of Tropical Crop Science Vol, 3(3).
Nandane, A.S., Dave, R.K. and Rao, T.R. 2017. Optimization of edible coating formulations for improving postharvest quality and shelf life of pear fruit using response surface methodology. Journal of Food Science and Technology, 54(1): 1-8.
Newerli-Guz, J., Śmiechowska, M., Drzewiecka, A. and Tylingo, R. 2023. Bioactive ingredients with health-promoting properties of strawberry fruit (Fragaria x ananassa Duchesne). Molecules, 28(6): 2711.
Oroojalian, F., Kasra-Kermanshahi, R., Azizi, M. and Bassami, M.R. 2010. Phytochemical composition of the essential oils from three Apiaceae species and their antibacterial effects on food-borne pathogens. Food Chemistry, 120(3): 765-770.
Oyom, W., Xu, H., Liu, Z., Long, H., Li, Y., Zhang, Z., Bi, Y., Tahergorabi, R. and Prusky, D. 2022. Effects of modified sweet potato starch edible coating incorporated with cumin essential oil on storage quality of ‘early crisp’. LWT, 153: 112475.
Paudel, S.K., Bhargava, K. and Kotturi, H. 2019. Antimicrobial activity of cinnamon oil nanoemulsion against Listeria monocytogenes and Salmonella spp. on melons. LWT, 111: 682-687.
Perez-Vazquez, A., Barciela, P., Carpena, M. and Prieto, M.A. 2023. Edible coatings as a natural packaging system to improve fruit and vegetable shelf life and quality. Foods, 12(19): 3570.
Petriccione, M., Mastrobuoni, F., Pasquariello, M.S., Zampella, L., Nobis, E., Capriolo, G. and Scortichini, M. 2015. Effect of chitosan coating on the postharvest quality and antioxidant enzyme system response of strawberry fruit during cold storage. Foods, 4(4): 501-523.
Peyromousavi, M., Kashiri, M., Maghsoudlou, Y., Komeiri, M. and Alami, M. 2018. Investigation the effect of type and plasticizer concentration on characteristic of novel biodegradable film based fine wheat powder. Iranian Journal of Food Science and Technology, 15(85): 33-47.
Piechowiak, T. and Skóra, B. 2023. Edible coating enriched with cinnamon oil reduces the oxidative stress and improves the quality of strawberry fruit stored at room temperature. Journal of the Science of Food and Agriculture, 103(5): 2389-2400.
Pinzon, M.I., Sanchez, L.T., Garcia, O.R., Gutierrez, R., Luna, J.C. and Villa, C.C. 2020. Increasing shelf life of strawberries (Fragaria ssp) by using a banana starch-chitosan Aloe vera gel composite edible coating. International Journal of Food Science and Technology, 55(1): 92-98.
Raeisi, M., Tajik, H., Yarahmadi, A. and Sanginabadi, S. 2015. Antimicrobial effect of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Health Scope, 4(4).
Resende, N.S., Gonçalves, G.A.S., Reis, K.C., Tonoli, G.H.D. and Boas, E.V.B.V. 2018. Chitosan/cellulose nanofibril nanocomposite and its effect on quality of coated strawberries. Journal of Food Quality, 2018(1): 1727426.
Saki, M., ValizadehKaji, B., Abbasifar, A. and Shahrjerdi, I. 2019. Effect of chitosan coating combined with thymol essential oil on physicochemical and qualitative properties of fresh fig (Ficus carica L.) fruit during cold storage. Journal of Food Measurement and Characterization, 13(2): 1147-1158.
Sapper, M., Palou, L., Pérez-Gago, M.B. and Chiralt, A. 2019. Antifungal starch–gellan edible coatings with thyme essential oil for the postharvest preservation of apple and persimmon. Coatings, 9(5): 333.
Schmidt, E., Jirovetz, L., Buchbauer, G., Eller, G.A., Stoilova, I., Krastanov, A., Stoyanova, A. and Geissler, M. 2006. Composition and antioxidant activities of the essential oil of cinnamon (Cinnamomum zeylanicum Blume) leaves from Sri Lanka. Journal of Essential Oil Bearing Plants, 9(2): 170-182.
Shiri, M.A., Ghasemnezhad, M., Bakhshi, D. and Saadatian, M. 2011. Effects of ascorbic acid on phenolic compounds and antioxidant activity of packaged fresh cut table grapes. Electronic Journal of Environmental, Agricultural and Food Chemistry, 10(7).
Singleton, V.L. and Rossi, J.A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3): 144-158.
Solís-Contreras, G.A., Rodríguez-Guillermo, M.C., de la Luz Reyes-Vega, M., Aguilar, C.N., Rebolloso-Padilla, O.N., Corona-Flores, J., de Abril Alexandra Soriano-Melgar, L. and Ruelas-Chacon, X. 2021. Extending shelf-life and quality of minimally processed golden delicious apples with three bioactive coatings combined with cinnamon essential oil. Foods, 10(3): 597.
Sun, T., Powers, J.R. and Tang, J. 2007. Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chemistry, 105(1): 101-106.
Wong, Y.C., Ahmad-Mudzaqqir, M.Y. and Wan-Nurdiyana, W.A. 2014. Extraction of essential oil from cinnamon (Cinnamomum zeylanicum). Oriental Journal of Chemistry, 30(1): 37.
Wongmetha, O. and Ke, L.S. 2012. The quality maintenance and extending storage life of mango fruit after postharvest treatments. World Academic of Science Engineering and Technology, 6(9): 844-9.
Xing, Y., Lin, H., Cao, D., Xu, Q., Han, W., Wang, R., Che, Z. and Li, X. 2015. Effect of chitosan coating with cinnamon oil on the quality and physiological attributes of China jujube fruits. BioMed Research International, 2015(1): 835151.
Xu, J., Zhou, L., Miao, J., Yu, W., Zou, L., Zhou, W., Liu, C. and Liu, W. 2020. Effect of cinnamon essential oil nanoemulsion combined with ascorbic acid on enzymatic browning of cloudy apple juice. Food and Bioprocess Technology, 13(5): 860-870.
Yang, F.M., Li, H.M., Li, F., Xin, Z.H., Zhao, L.Y., Zheng, Y.H. and Hu, Q.H. 2010. Effect of nano‐packing on preservation quality of fresh strawberry (Fragaria ananassa Duch. cv Fengxiang) during storage at 4 C. Journal of Food Science, 75(3): C236-C240.
Yin, C., Huang, C., Wang, J., Liu, Y., Lu, P. and Huang, L. 2019. Effect of chitosan-and alginate-based coatings enriched with cinnamon essential oil microcapsules to improve the postharvest quality of mangoes. Materials, 12(13): 2039.
Yousuf, B., Qadri, O.S. and Srivastava, A.K. 2018. Recent developments in shelf-life extension of fresh-cut fruits and vegetables by application of different edible coatings: A review. Lwt, 89: 198-209.
Yu, K., Xu, J., Zhou, L., Zou, L. and Liu, W. 2021. Effect of chitosan coatings with cinnamon essential oil on postharvest quality of mangoes. Foods, 10(12): 3003.
Zhang, Y., Ma, Q., Critzer, F., Davidson, P.M. and Zhong, Q. 2015. Effect of alginate coatings with cinnamon bark oil and soybean oil on quality and microbiological safety of cantaloupe. International Journal of Food Microbiology, 215: 25-30.
Zhou, Y., Hu, L., Chen, Y., Liao, L., Li, R., Wang, H., Mo, Y., Lin, L. and Liu, K. 2022. The combined effect of ascorbic acid and chitosan coating on postharvest quality and cell wall metabolism of papaya fruits. LWT, 171: 114134.