Response to salinity stress in Guava seedling genotypes

Document Type : Original Article

Authors

1 Msc. graduated, University of Hormozgan, Bandar Abbas. Iran.

2 Associate professor, Faculty of Agriculture, University of Hormozgan, Bandar Abbas. Iran.

3 Assistant Professor, Horticulture Crops Research Department, Qazvin Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Qazvin, Iran.

10.30466/rip.2023.54612.1272

Abstract

Selection of salt-tolerant genotypes is an effective approach for dealing with saline water and soils. A greenhouse experiment was conducted from 2016 to 2018 in the greenhouse of the Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan, to evaluate the morphological, physiological, and biochemical responses of guava seedlings to saline water. Twelve one-year-old seed-derived guava genotypes were irrigated with saline water (0.5 and 5 dS m⁻¹) for ten weeks. Treatment with saline water resulted in reductions in leaf relative water content, chlorophyll pigments, carotenoids, protein, anthocyanins, photosystem II photochemical efficiency, and shoot and leaf dry matter in guava genotypes. In addition, electrolyte leakage and the contents of soluble sugars, as well as the activities of protease, catalase, and peroxidase enzymes, showed increasing trends. The highest leaf and shoot dry matter at a salinity level of 5 dS m⁻¹ was observed in genotypes AC-04 and AC-05, respectively. The lowest leaf dry matter was recorded in genotypes AC-01, AC-02, AC-03, and AC-11 at 5 dS m⁻¹ salinity. The lowest shoot dry matter was observed in genotypes AC-02, AC-06, and AC-08 (49.76%, 50.60%, and 51.12%, respectively). Cluster analysis divided the genotypes into four groups. In the first group (AC-11, AC-10, and AC-12), the lowest levels of protein, peroxidase activity, and photosystem II photochemical efficiency, along with the highest ion leakage, were recorded. In the second group (AC-02, AC-01, and AC-03), the lowest levels of photosynthetic pigments and catalase activity were observed. The third group (AC-08, AC-07, AC-06, and AC-09) exhibited an intermediate response. Genotypes of AC-04 and AC-05 formed the fourth group, characterized by the highest leaf relative water content, pigment concentration, catalase and peroxidase activities, and leaf dry matter. Ultimately, AC-04 and AC-05 were identified as salt-tolerant genotypes, while AC-11, AC-10, and AC-12 were classified as salt-sensitive genotypes.

Keywords

References

بی­نام. 1400. آمارنامه کشاورزی. محصولات باغبانی (جلد 3) کتاب: وزارت جهاد کشاورزی، معاونت برنامه‌ریزی و اقتصادی، مرکز فناوری اطلاعات و ارتباطات.
پشنگه، ز.، شمیلی، م.، عبدالهی، ف. و قاسمی، م. 1399. برهمکنش شوری و جیبرلین بر ریزش برگ، ماده خشک، فعالیت     آنزیم­های آنتی­اکسیدان و محتوای عناصر در گواوا (Psidium guajava L.). مجله پژوهش­های گیاهی (انجمن زیست­شناسی ایران)، 3(4): 826-809.
پوری، ن.، سیفی، ا. و علیزاده، م. 1396. ارزیابی اثر تنش شوری و پرولین بر برخی از صفات ریخت­شناختی، فیزیکی و فیتوشیمیایی برگ در سه رقم زیتون (Olea europaea L.). فصلنامه اکوفیتوشیمی گیاهان دارویی، 5(2): 69-85.
جلیلی­مرندی، ر.، حسنی، ع.، دولتی­بانه، ح.، حاجی­تقی­لو، ر. و یوسف­زاده، ح. 1391. تاثیر سطوح شوری بر ویژگی­های مورفولوژیکی و فیزیولوژیکی دو رقم انگور. نشریه علوم باغبانی، 26(1): 68-77.
بیبی، ف. و امیری، م.ا. 1392. بررسی فعالیت آنزیمی و واکنش­های بیوشیمیایی دو پایة مرکبات به تنش شوری درون شیشه­ای. به­زراعی کشاورزی، 15(4): 165-177.
خواجه، ر.، قاسمی، م.، میرزاعلیان­دستجردی، ع. و دمی­زاده، غ. 1400. پاسخ دانهال­های مکزیکن­لایم(Citrus aurantifolia cv. Mexican lime) به اسید سالیسیلیک در شرایط تنش شوری، 6(1): 10-21.
سوری، ن.، بخشی، د.، رضایی­نژاد، ع. و فیضیان، م. 1398. اثر تنش شوری بر برخی ویژگی­های فیزیولوژیک و پارامترهای فتوسنتزی چند ژنوتیپ تجاری انار ایرانی. فرآیند و کارکرد گیاهی، 8(30): 170-155.
عطار­زاده، م.، رحیمی، ا و ترابی، ب. 1395. واکنش کلروفیل، محتوای نسبی آب و پروتئین برگ گلرنگ به تنش شوری و محلول‌پاشی کلسیم، پتاسیم و منگنز. نشریه علمی-پژوهشی اکوفیزیولوژی گیاهان زراعی، 10، 1(37): 282-269.
فاضلی، آ.، زارعی، ب. و طهماسبی، ز.1396. تأثیر تنش شوری و سالیسیلیک اسید بر برخی ویژگی­های فیزیولوژیک و بیوشیمیایی سیاه دانه (Nigella sativa L.). زیست­شناسی گیاهی ایران، 9(34): 83-69.
فلاح، ا.، فرهمند­فر، ا. و مرادی، ف. 1394. تأثیر تنش شوری در مراحل مختلف رشد بر برخی صفات مورفوفیزیولوژیکی دو رقم برنج در شرایط گلخانه. مجله پژوهش­های کاربردی زراعی،  28(107): 182-175.
محمودیه چم­پیری، ر. و ابوطالبیان، م.ع. 1400. بررسی خصوصیات فیزیولوژیک دو رقم گندم (Triticum aestivum) در پاسخ به تنش شوری و مایکوریزا. نشریه علوم گیاهان زراعی ایران، 52(4): 73-86.
مومن­پور، ع. و ایمانی، ع. . 1398. اثر تنش شوری بر ویژگی­های رشدی تعدادی از ژنوتیپ­های انتخابی بادام. نشریه پژوه­­های تولید گیاهی، 26(2): 29-46.
مومن­پور، ع.، ایمانی، ع. و بخشی، د. 1397. تأثیر تنش شوری بر برخی از صفات بیوشیمیایی چهار رقم بادام. نشریه پژوهش آب در کشاورزی، 32(2): 201-216.
Abbasi, J.A., Gandahi, A.W., Saleem, M., Bhatti, S.M. and Bughio, Z.R. 2019. Growth and development of guava (Psidium guajava l.) seedlings against sodium chloride salinized water: Department of Soil Science, Sindh Agriculture University Tandojam, Pakistan. Pakistan Journal of Agriculture, Agricultural Engineering and Veterinary Sciences, 35(2): 81-85.
Ahmad, P., Ozturk, M., Sharma, S. and Gucel, S. 2014. Effect of sodium carbonate-induced salinity–alkalinity on some key osmoprotectants, protein profile, antioxidant enzymes, and lipid peroxidation in two mulberry (Morus alba L.) cultivars. Journal of Plant Interactions, 9(1): 460-467.
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24(1): 1.
Asada, K. 2006. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiology, 141(2): 391-396.
Ben Amor, N., Megdiche, W., Jiménez, A., Sevilla, F. and Abdelly, C. 2010. The effect of calcium on the antioxidant systems in the halophyte Cakile maritima under salt stress. Acta Physiologiae Plantarum, 32: 453-461.
Bezerra, I.L., Nobre, R.G., Gheyi, H.R., Lima, G.S.D. and Barbosa, J.L. 2018. Physiological indices and growth of ‘Paluma’guava under saline water irrigation and nitrogen fertigation. Revista Caatinga, 31(04): 808-816.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254.
Cavalcante, Í.H.L., Cavalcante, L.F., Hu, Y. and Beckmann-Cavalcante, M.Z. 2007. Water salinity and initial development of four guava (Psidium guajava L.) cultivars in north-eastern Brazil. Journal of Fruit and Ornamental Plant Research, (15): 71-80.
Cavalcante, L.F., Vieira, M.D.S., Santos, A.F.D., Oliveira, W.M.D. and Nascimento, J.A.M.D. 2010. Água salina e esterco bovino líquido na formação de mudas de goiabeira cultivar Paluma. Revista Brasileira de Fruticultura, 32: 251-261.
Change, B. and Maehly, A.C. 1955. Assay of catalases and peroxidase. Methods Enzymol, 2: 764-775.
Chen, M., Zhao, Y. and Yu, S. 2015. Optimisation of ultrasonic-assisted extraction of phenolic compounds, antioxidants, and anthocyanins from sugar beet molasses. Food Chemistry, 172: 543-550.
de Oliveira, V.P., Marques, E.C., de Lacerda, C.F., Prisco, J.T. and Gomes Filho, E. 2013. Physiological and biochemical characteristics of Sorghum bicolor and Sorghum sudanense subjected to salt stress in two stages of development. African Journal of Agricultural Research, 8(8): 660-670.
Dexter, S.T., Tottingham, W.E. and Graber, L.F. 1932. Investigations of the hardiness of plants by measurement of electrical conductivity. Plant Physiology, 7(1): 63.
Dhindsa, R.S. and Matowe, W. 1981. Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany, 32(1): 79-91.
Dichala, O., Therios, I., Papadopoulos, A., Chatzistathis, T., Chatzisavvidis, C. and Antonopoulou, C., 2021. Effects of varying concentrations of different salts on mineral composition of leaves and roots of three pomegranates (Punica granatum L.) cultivars. Scientia Horticulturae, 275: 109718.
Doganlar, ZB., Demir, K., Basak, H. and Gul, I. 2010. Effects of salt stress on pigment and total soluble protein contents of three different tomato cultivars. African Journal Agriculture Research. 5(15): 2056-2065.
Ebert, G. 1999. Salinity problems in sub-tropical fruit production. Acta Horticulture, 53 (1): 99-105.
Emamverdian, A., Ding, Y. and Mokhberdoran, F. 2020. The role of salicylic acid and gibberellin signaling in plant responses to abiotic stress with an emphasis on heavy metals. Plant Signaling and Behavior, 15(7): 1777372.
Erturk, U., Sivritepe, N., Yerlikaya, C., Bor, M., Ozdemir, F. and Turkan, I. 2007. Responses of the cherry rootstock to salinity in vitro. Biologia Plantarum, 51: 597-600.
Garratt, L.C., Janagoudar, B.S., Lowe, K.C., Anthony, P., Power, J.B. and Davey, M.R. 2002. Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine, 33(4): 502-511.
Gomes, K.R., Amorim, A.V., Ferreira, F.J., A Filho, F.L., Lacerda, C.F. and Gomes-Filho, E. 2011. Physiology and growth responses of maize subjected to salt stress in different cultiving spacings. Revista Brasileira de Engenharia Agrícola e Ambiental, 15(4): 365-370.
Gupta, B. and Huang, B. 2014. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International Journal of Genomics, 2014(1): 701596.
Hajheidari, M., Abdollahian‐Noghabi, M., Askari, H., Heidari, M., Sadeghian, S.Y., Ober, E.S. and Hosseini Salekdeh, G. 2005. Proteome analysis of sugar beet leaves under drought stress. Proteomics, 5(4): 950-960.
He, Y. and Zhu, Z.J. 2008. Exogenous salicylic acid alleviates NaCl toxicity and increases antioxidative enzyme activity in Lycopersicon esculentum. Biologia Plantarum, 52: 792-795.
Ibrahim, H.I. 2016. Tolerance of two pomegranates cultivars (Punica granatum L.) to salinity stress under hydroponic culture conditions. Journal of Basic and Applied Scientific Research, 6(4): 38-46.
Ji, X., Tang, J. and Zhang, J. 2022. Effects of salt stress on the morphology, growth and physiological parameters of Juglans microcarpa L. seedlings. Plants, 11(18): 2381.
Jithesh, M.N., Prashanth, S.R., Sivaprakash, K.R. and Parida, A.K. 2006. Antioxidative response mechanisms in halophytes: their role in stress defence. Journal of Genetics, 85: 237-254.
Joshi, S. and Satyanarayana, T. 2013. Characteristics and applications of a recombinant alkaline serine protease from a novel bacterium Bacillus lehensis. Bioresource Technology, 131: 76-85.
Kaya, C., Higgs, D. and Kirnak, H. 2001. The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Bulg. Journal of Plant Physiology, 27(3-4): 47-59.
Lu, S., Li, T. and Jiang, J. 2010. Effects of salinity on sucrose metabolism during tomato fruit development. African journal of Biotechnology, 9(6): 842-849.
Ma, Y., Wei, Z., Liu, J., Liu, X. and Liu, F. 2021. Growth and physiological responses of cotton plants to salt stress. Journal of Agronomy and Crop Science, 207(3): 565-576.
Marques, A.M., Tuler, A.C., Carvalho, C.R., Carrijo, T.T., da Silva Ferreira, M.F. and Clarindo, W.R. 2016. Refinement of the karyological aspects of Psidium guineense (Swartz, 1788): a comparison with Psidium guajava (Linnaeus, 1753). Comparative Cytogenetics, 10(1): 117.
McCready, R.M., Guggolz, J., Silviera, V. and Owens, H.S. 1950. Determination of starch and amylose in vegetables. Analytical Chemistry, 22(9): 1156-1158.
Michalak, A., 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4): 523-530.
Misra, A.N., Misra, M. and Singh, R. 2012. Chlorophyll fluorescence in plant biology. Biophysics, 7: 171-192.
Molassiotis, A.N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G. and Therios, E. 2006. Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCl, mannitol or sorbitol. Biologia Plantarum, 50: 331-338.
Ndoumou, D.O., Djocgoue, P.F., Nana, L. and Debost, M., 1995. Variation and inheritance of peroxidase activity and phenol and saccharide content in cacao in relation to susceptibility to black pod disease. Biologia Plantarum, 37: 429-436.
Neocleous, D. and Vasilakakis, M. 2007. Effects of NaCl stress on red raspberry (Rubus idaeus L. ‘Autumn Bliss’). Scientia Horticulturae, 112(3): 282-289.
Nuccio, M.L., Rhodes, D., McNeil, S.D. and Hanson, A.D. 1999. Metabolic engineering of plants for osmotic stress resistance. Current Opinion in Plant Biology, 2(2): 128-134.
Parida, A.K. and Das, A.B. 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60(3): 324-349.
Parida, A.K., Das, A.B., Mittra, B. and Mohanty, P. 2004. Salt-stress induced alterations in protein profile and protease activity in the mangrove Bruguiera parviflora. Zeitschrift für Naturforschung C, 59(5-6): 408-414.
Parvaiz, A. and Satyawati, S. 2008. Salt stress and phyto-biochemical responses of plants-a review. Plant Soil and Environment, 54(3): 89.
Belkhodja, R., Morales, F., Abadia, A., Gomez-Aparisi, J. and Abadia, J. 1994. Chlorophyll fluorescence as a possible tool for salinity tolerance screening in barley (Hordeum vulgare L.). Plant Physiology, 104(2): 667-673.
Raya, A.M., Ruiz, J.A., Zuazo, V.H.D. and Tarifa, D.F. 2004. Impact of salinity on macro-and micronutrient uptake in mango (Mangifera indica L. cv. Osteen) with different rootstocks. Spanish Journal of Agricultural Research, 2(1): 121-134.
Ritchie, S.W., Nguyen, H.T. and Holaday, A.S. 1990. Leaf water content and gas‐exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30(1): 105-111.
Sá, F.V.D.S., Nobre, R.G., Silva, L.D.A., Moreira, R.C., Paiva, E.P.D. and Oliveira, F.A.D. 2016. Tolerance of guava rootstocks under salt stress. Revista Brasileira de Engenharia Agrícola e Ambiental, 20(12): 1072-1077.
Shamili, M and Hormaza, J.I. 2021. Genotypic and phenotypic diversity in guava (Psidium guajava L.) genotypes from Iran. Fruits. 76(1): 11–21.
Sofo, A., Dichio, B., Xiloyannis, C. and Masia, A. 2005. Antioxidant defences in olive trees during drought stress: changes in activity of some antioxidant enzymes. Functional Plant Biology, 32(1): 45-53.
Stępień, P. and Kłbus, G. 2006. Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress. Biologia Plantarum, 50(4): 610-616.
Strasser, R.J., Srivastava, A. and Tsimilli-Michael, M. 2000. The fluorescence transient as a tool to characterize and screen photosynthetic samples. Probing Photosynthesis: Mechanisms, Regulation and Adaptation, 25: 445-483.
Sudhir, P. and Murthy, S.D.S. 2004. Effects of salt stress on basic processes of photosynthesis. Photosynthetica, 42(4): 481-486.
Sývacý, A. and Sökmen, M. 2004. Seasonal changes in antioxidant activity, total phenolic and anthocyanin constituent of the stems of two Morus species (Morus alba L. and Morus nigra L.). Plant Growth Regulation, 44: 251-256.
Tate, D. 2000. Tropical Fruit of Thailand (Bangkok, Thailand: Asia Books Co. Ltd.), 96 p.
Thaipong, K. and Boonprakob, U. 2019. Salt tolerance evaluation in guava germplasm. International Journal of Agricultural Technology, 15(5): 791-796.
Wahid, A. and Ghazanfar, A. 2006. Possible involvement of some secondary metabolites in salt tolerance of sugarcane. Journal of Plant Physiology, 163(7): 723-730.
Wang, Y., Loake, G.J. and Chu, C. 2013. Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death. Frontiers in Plant Science, 4: 314.
Zhao, H., Liang, H., Chu, Y., Sun, C., Wei, N., Yang, M. and Zheng, C. 2019. Effects of salt stress on chlorophyll fluorescence and the antioxidant system in Ginkgo biloba L. seedlings. HortScience, 54(12): 2125-2133.
Zhong, Y.P., Qi, X.J., Chen, J.Y., Zhi, L.I., Bai, D.F., Wei, C.G. and Fang, J.B. 2019. Growth and physiological responses of four kiwifruit genotypes to salt stress and resistance evaluation. Journal of Integrative Agriculture, 18(1): 83-95.
Zhu, J.K., 2007. Plant salt stress. Encyclopedia of Life Sciences, 2: 1-7.
Zhu, S., Nong, J., Luo, G., Li, Q., Wang, F., Jiang, D. and Zhao, X. 2021. Varied tolerance and different responses of five citrus rootstocks to acid stress by principle component analysis and orthogonal analysis. Scientia Horticulturae, 278: 109853.
Research in Pomology
Volume 9, Issue 1
November 2024
Pages 204-225

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